Phenexplain Demonstration

In this example, training was performed on images obtained in the following way. IPSCs were derived from fibroblasts sampled from a LRRK2 G2019S mutated Parkinson's patient. These IPSCs were then reprogrammed to dopaminergic neurons with or without a CRISPR-cas9 correction of the G2019S mutation. The last is then an isogenic engineered wild type. Large sets of confocal images with one dye labeling for Nuclei, and antibody stains labeling for alpha synuclein and TH cells were acquired and displayed no visible differences. Using our approach, we can clearly see that to transform mutated neuron to rescued wild type, the system needs to remove a part of the IPS cells and transfom some others into neurons. All displayed imaged are synthetic.